The NeuroLINCS Center creates a wealth of cellular data that is patient-specific in the context of their baseline genetic perturbations and in the presence of other environmental stressors. Motor neurons (iMNs) are produced from induced pluripotent stem cell (iPSC) technologies and these cells are analyzed using epigenomics, transcriptomics, proteomics, and robotic imaging.
Amyotrophic lateral sclerosis (ALS) is a severe neurodegenerative condition characterized by loss of motor neurons in the brain and spinal cord. Spinal muscular atrophy (SMA) is a recessively inherited pediatric neuromuscular disease characterized by degeneration of spinal motor neurons. The control (CTR) cells come from healthy individuals.
iPSC - ALS, SMA and Control (unaffected) subject-derived iPSC lines.
WGS, RNA-Seq, ATAC-Seq and SWATH-MS were carried out on isolated mRNA, DNA and protein respectively obtained from induced Pluripotent Stem Cell (iPSC) lines. These lines were derived from ALS, SMA and Control (unaffected) individuals. Multiple levels of data and analysis including full information on the number of replicates are available at the links below.
Motor Neurons (iMNs) – ALS, SMA and Control (unaffected) subject motor neuron lines
iMN long differentiation protocol
Motor Neurons (diMNs) – ALS and Control (unaffected) subject motor neuron lines
diMN short differentiation protocol - mature neurons